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Table 6 Quantification of amino acids present in model sample of Waters amino acid standard hydrolysate

From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis

  Expected concentration [µM] AA standard hydrolysate [µM] Recovery [%]
Glycine 250 249.35 ± 3.04 99.7
Alanine 250 255.85 ± 4.17 102.3
Serine 250 245.45 ± 3.18 98.2
Proline 250 256.85 ± 3.47 102.7
Valine 250 237.25 ± 3.47 94.9
Threonine 250 251.25 ± 4.31 100.5
Cysteine 125 125.05 ± 3.89 100.0
Isoleucine 250 255.10 ± 4.10 102.0
Leucine 250 244.45 ± 1.49 97.8
Aspartic acid 250 239.75 ± 1.34 95.9
Lysine 250 245.60 ± 2.12 98.2
Glutamic acid 250 227.57 ± 10.43 91.0
Methionine 250 247.90 ± 3.96 99.2
Histidine 250 260.60 ± 1.27 104.2
Phenylalanine 250 237.75 ± 0.64 95.1
Arginine 250 246.00 ± 2.12 98.4
Tyrosine 250 236.5 ± 3.54 94.6