Table 6 Quantification of amino acids present in model sample of Waters amino acid standard hydrolysate
From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis
Expected concentration [µM] | AA standard hydrolysate [µM] | Recovery [%] | |
|---|---|---|---|
Glycine | 250 | 249.35 ± 3.04 | 99.7 |
Alanine | 250 | 255.85 ± 4.17 | 102.3 |
Serine | 250 | 245.45 ± 3.18 | 98.2 |
Proline | 250 | 256.85 ± 3.47 | 102.7 |
Valine | 250 | 237.25 ± 3.47 | 94.9 |
Threonine | 250 | 251.25 ± 4.31 | 100.5 |
Cysteine | 125 | 125.05 ± 3.89 | 100.0 |
Isoleucine | 250 | 255.10 ± 4.10 | 102.0 |
Leucine | 250 | 244.45 ± 1.49 | 97.8 |
Aspartic acid | 250 | 239.75 ± 1.34 | 95.9 |
Lysine | 250 | 245.60 ± 2.12 | 98.2 |
Glutamic acid | 250 | 227.57 ± 10.43 | 91.0 |
Methionine | 250 | 247.90 ± 3.96 | 99.2 |
Histidine | 250 | 260.60 ± 1.27 | 104.2 |
Phenylalanine | 250 | 237.75 ± 0.64 | 95.1 |
Arginine | 250 | 246.00 ± 2.12 | 98.4 |
Tyrosine | 250 | 236.5 ± 3.54 | 94.6 |