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Table 2 Data obtained from the optimization procedure of the stacionary phase

From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis

  Kinetex C18 Acquity UPLC HSS T3 Cortecs UPLC C18
RT (min) w1/2 (min) N Resolution Ile-Leu RT (min) w1/2 (min) N Resolution Ile-Leu RT (min) w1/2 (min) N Resolution Ile-Leu
Gly 1.97 0.03 23,889   2.26 0.03 31,440   2.06 0.0295 27,015  
Ala 2.27 0.03 31,719   2.68 0.03 44,212   2.56 0.0295 41,720  
Ser 1.91 0.03 22,456   2.17 0.04 16,305   1.99 0.02 54,847  
Pro 2.44 0.035 26,925   2.96 0.04 30,337   2.91 0.02 117,283  
Val 3.58 0.05 28,401   4.68 0.045 59,920   4.60 0.03 130,252  
Thr 2.13 0.03 27,927   2.45 0.025 53,206   2.33 0.025 48,122  
Cys 2.87 0.03 50,703   3.50 0.035 55,400   4.13 0.03 104,995  
Ile 4.97 0.045 67,577 1.49 5.71 0.04 112,892 1.52 5.49 0.03 185,529 1.72
Leu 5.09 0.05 57,412 5.80 0.03 207,073 5.57 0.025 275,005
Asn 1.81 0.03 20,166   2.08 0.025 38,349   1.89 0.025 31,663  
Asp 2.00 0.035 18,090   2.28 0.025 46,079   2.13 0.025 40,215  
Gln 1.90 0.035 16,326   2.14 0.025 40,594   2.00 0.02 55,400  
Glu 2.08 0.03 26,631   2.35 0.03 33,994   2.24 0.02 69,494  
Lys 3.03 0.035 41,520   3.68 0.035 61,244   4.24 0.03 110,662  
Met 3.47 0.035 54,454   4.50 0.04 70,116   4.47 0.03 122,994  
His 1.71 0.025 25,919   1.98 0.025 34,750   1.79 0.02 44377  
Phe 5.30 0.05 62,247   5.97 0.025 315,921   5.72 0.03 201,400  
Arg 1.90 0.03 22,222   2.11 0.03 27,405   1.96 0.02 53,206  
Tyr 3.31 0.05 24,279   4.20 0.04 61,079   4.36 0.035 147,967  
Trp 5.50 0.06 46,551   6.11 0.03 229,800   5.84 0.025 302,312