From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis
Kinetex C18 | Acquity UPLC HSS T3 | Cortecs UPLC C18 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
RT (min) | w1/2 (min) | N | Resolution Ile-Leu | RT (min) | w1/2 (min) | N | Resolution Ile-Leu | RT (min) | w1/2 (min) | N | Resolution Ile-Leu | |
Gly | 1.97 | 0.03 | 23,889 | 2.26 | 0.03 | 31,440 | 2.06 | 0.0295 | 27,015 | |||
Ala | 2.27 | 0.03 | 31,719 | 2.68 | 0.03 | 44,212 | 2.56 | 0.0295 | 41,720 | |||
Ser | 1.91 | 0.03 | 22,456 | 2.17 | 0.04 | 16,305 | 1.99 | 0.02 | 54,847 | |||
Pro | 2.44 | 0.035 | 26,925 | 2.96 | 0.04 | 30,337 | 2.91 | 0.02 | 117,283 | |||
Val | 3.58 | 0.05 | 28,401 | 4.68 | 0.045 | 59,920 | 4.60 | 0.03 | 130,252 | |||
Thr | 2.13 | 0.03 | 27,927 | 2.45 | 0.025 | 53,206 | 2.33 | 0.025 | 48,122 | |||
Cys | 2.87 | 0.03 | 50,703 | 3.50 | 0.035 | 55,400 | 4.13 | 0.03 | 104,995 | |||
Ile | 4.97 | 0.045 | 67,577 | 1.49 | 5.71 | 0.04 | 112,892 | 1.52 | 5.49 | 0.03 | 185,529 | 1.72 |
Leu | 5.09 | 0.05 | 57,412 | 5.80 | 0.03 | 207,073 | 5.57 | 0.025 | 275,005 | |||
Asn | 1.81 | 0.03 | 20,166 | 2.08 | 0.025 | 38,349 | 1.89 | 0.025 | 31,663 | |||
Asp | 2.00 | 0.035 | 18,090 | 2.28 | 0.025 | 46,079 | 2.13 | 0.025 | 40,215 | |||
Gln | 1.90 | 0.035 | 16,326 | 2.14 | 0.025 | 40,594 | 2.00 | 0.02 | 55,400 | |||
Glu | 2.08 | 0.03 | 26,631 | 2.35 | 0.03 | 33,994 | 2.24 | 0.02 | 69,494 | |||
Lys | 3.03 | 0.035 | 41,520 | 3.68 | 0.035 | 61,244 | 4.24 | 0.03 | 110,662 | |||
Met | 3.47 | 0.035 | 54,454 | 4.50 | 0.04 | 70,116 | 4.47 | 0.03 | 122,994 | |||
His | 1.71 | 0.025 | 25,919 | 1.98 | 0.025 | 34,750 | 1.79 | 0.02 | 44377 | |||
Phe | 5.30 | 0.05 | 62,247 | 5.97 | 0.025 | 315,921 | 5.72 | 0.03 | 201,400 | |||
Arg | 1.90 | 0.03 | 22,222 | 2.11 | 0.03 | 27,405 | 1.96 | 0.02 | 53,206 | |||
Tyr | 3.31 | 0.05 | 24,279 | 4.20 | 0.04 | 61,079 | 4.36 | 0.035 | 147,967 | |||
Trp | 5.50 | 0.06 | 46,551 | 6.11 | 0.03 | 229,800 | 5.84 | 0.025 | 302,312 |