Fig. 2

Response surface plots representing relationship between % delignification and a incubation time and pH b pH and enzyme concentration c incubation time and enzyme concentration d incubation time and temperature. Raw pineapple leaf waste powder is the substrate. Effect of interaction between two variables such as incubation time (5–7 h) and pH (6–8), enzyme concentration (300–700 IU/mL) and pH (6–8), incubation time (5–7 h) and enzyme concentration (300–700 IU/mL), incubation time (5–7 h) and temperature (35–45 °C) on the delignification (%) has been studied