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Fig. 5 | BMC Chemistry

Fig. 5

From: Synthesis and evaluation of novel naphthol diazenyl scaffold based Schiff bases as potential antimicrobial and cytotoxic agents against human colorectal carcinoma cell line (HT-29)

Fig. 5

Dot plot (a untreated; b standard;  c NS-2; d NS-21) which refers all the cells took for the analysis and selected some cells in the centre of this plot called as gated cells (selected region) R1-Region 1 since those cells are single cells and cells of our interest. Above the gated region cells are clumped cells or may be double or triple clump of cells and below the gated region cells are debris or dead cells. Cells based on the cells size and granularity will appear in the dot plot where FSC—Forward scattering light refers granularity and shape of cells and SSC—Side Scattering light refers size of the cells

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