Evaluating urinary estrogen and progesterone metabolites using dried filter paper samples and gas chromatography with tandem mass spectrometry (GC–MS/MS)

Table 2 Comparison of urinary hormone metabolites measured with the dried urine assay to serum hormones concentrations measured by RIA

	Urinary estradiol (ng/mg-Cr) v serum estradiol (pg/ml)	Urinary estrone (ng/mg-Cr) v serum estradiol (pg/ml)	Urinary αPg (ng/mg-Cr) v serum progesterone (ng/ml)	Urinary βPg (ng/mg-Cr) v serum progesterone (ng/ml)
Dried urine assay	0.39 (0.11, 1.87)	7.47 (2.67, 13.44)	21.34 (13.54, 49.48)	97.92 (58.47, 174.01)
Serum assay	15.38 (7.84, 91.90)	15.38 (7.84, 91.90)	0.24 (0.08, 1.57)	0.24 (0.08, 1.57)
Standardized urine measures	− 0.05 (− 0.32, 0.46)	0.29 (− 0.26, 0.57)	0.89 (− 0.01, 0.34)	0.09 (− 0.20, 0.46)
Standardized serum measures	0.33 (0.22, 0.41)	0.33 (0.22, 0.41)	0.07 (− 0.05, 0.30)	0.07 (− 0.05, 0.30)
p-value for paired comparisons of standardized measures	0.47	0.99	0.32	0.03
ICC	0.78	0.83	0.83	0.85

Data presented as median (IQR). Standardization of the urine and serum measures was accomplished using the min–max scaling with established reference ranges. The p-values for the paired comparisons were obtained using mixed models to account for repeated measures
ICC, intraclass correlation coefficient; Cr, creatinine