Identification and characterization of in vivo, in vitro and reactive metabolites of vandetanib using LC–ESI–MS/MS

Table 3 Phase I metabolites of Vandetanib using MS scan and PI scan

	MS scan	Major daughter ions	t_R (min)	Metabolic pathway	Proposed conjugate composition	Previously detected (reference)
VNT	475	112	50.4		V + H
VA461	461	364, 98	49.7	N-demethylation	V − CH₂ + H	+ [1]
VA475	475	112, 110	54.7	N-demethylation and α oxidation	V − CH₂ + O + H
VA489a	489	126	66.8	α oxidation	V + O − 2H + H
VA489b	489	364	67.9	N-demethylation and 2 α oxidation	V − CH₂ + 2O + H
VA491a	491	128, 111	57.1	α Hydroxylation	V + O + H
VA491b	491	189, 128	50.4	N-oxidation	V + O + H	+ [1]

ISSN: 2661-801X