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Table 1 Online solid phase extraction (SPE) operating procedures

From: Simultaneous determination of five azadirachtins in the seed and leaf extracts of Azadirachta indica by automated online solid-phase extraction coupled with LC–Q-TOF–MS

Step

Operation

Solvent

Flow rate (µL min−1)

Volume (µL)

1

Activation

MeOH

5000

1000

2

Equilibration

H2O

5000

1000

3

Loading SPE

10:90 MeOH/H2O

700

700

4

Washing SPE

30:70 MeOH/H2O

5000

1000

5

Elution

MeOH

150

300

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BMC Chemistry

ISSN: 2661-801X

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