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Table 1 Online solid phase extraction (SPE) operating procedures

From: Simultaneous determination of five azadirachtins in the seed and leaf extracts of Azadirachta indica by automated online solid-phase extraction coupled with LC–Q-TOF–MS

Step Operation Solvent Flow rate (µL min−1) Volume (µL)
1 Activation MeOH 5000 1000
2 Equilibration H2O 5000 1000
3 Loading SPE 10:90 MeOH/H2O 700 700
4 Washing SPE 30:70 MeOH/H2O 5000 1000
5 Elution MeOH 150 300