Table 2 GC conditions used for analysis of PAHs in mainstream smoke

Injection

Multimode (PTV) injection, splitless mode

Injection volume

2 µl

Carrier gas

Helium; 1 ml/min (50 min), then 2 ml/min (until the end of the analytical run)

Column

Agilent DB-EUPAH (60 m × 250 mm × 0.25 µm)

Oven temperature programme

50 °C (0.8 min), ramp 45 °C/min to 200 °C, ramp 2.5 °C/min to 225 °C, ramp 5 °C/min to 266 °C, ramp 14 °C/min to 300 °C, ramp 10 °C/min up to 320 °C (48 min). Total run time 74.762 min