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Fig. 3 | Chemistry Central Journal

Fig. 3

From: Anti-inflammatory effect of oligostilbenoids from Vitis heyneana in LPS-stimulated RAW 264.7 macrophages via suppressing the NF-κB activation

Fig. 3

Comparison of COX-2 protein expression effects of compounds from V. heyneana. 18 h after treating cells with LPS (5 μg/mL) with or without compounds in RAW 264.7 cells, samples were harvested and lysated to immunoblottings with COX-2 and β-actin antibodies. b COX-2 mRNA level by qCPR (***significant as compared to control, *p < 0.05; #significant as compared to LPS group, n = 5. c Comparison of PGE2 production effects of all compounds (10 M). RAW 264.7 cells were incubated with 5 μg/mL LPS for 18 h with or without compounds and amount of PGE2 in medium was determined using PGE2-specific ELISA assays (***significant as compared to control, *p < 0.05; #significant as compared to LPS group, n = 5. Codes: VH02 compound 1; VH03 - 2; VH04 - 3; VH06 - 4; VH07 - 5; VH08 - 6; VH11 - 7; VH13 - 8; VH15 - 9; and VH16 - 10. 

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