Table 8 Comparison between the developed and the published HPLC methods
From: Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods
The method | The chromatographic condition |
|---|---|
The developed HPLC method | Stationary phase: CN column Mobile phase: acetonitrile: water (40:60, v/v) Flow rate: 1 mL/min Retention time: 8.96 min Linearity range: 2–50 µg/mL Detection wavelength: 220 nm |
Determination of flutamide in tablets [3] | Stationary phase: Luna C18 column Mobile phase: 0.05 M phosphate buffer (pH = 4): acetonitrile (50:50, v/v) Flow rate: 1 mL/min Retention time: 5.57 min Linearity range: 2.9–11.6 µg/mL |
HPLC method for flutamide in pure form and dosage form [7] | Stationary phase: Packing L1 column Mobile phase: Acetonitrile: water (55:45, v/v). Flow rate: 1 mL/min Detection wavelength: 240 nm Retention time: 1 min |
Stability study of flutamide [16] | Stationary phase: Licrospher RP-18. Mobile phase: Acetonitrile: water: methanol (30:45:25, by volume). Flow rate: 1 mL/min Detection wavelength: 299 nm |
Stability study of flutamide [17] | Stationary phase: C18 column Mobile phase: methanol: 0.04 M phosphate buffer (pH = 4) (75:25, v/v). Detection wavelength: 240 nm Retention time: 4 min Linearity range: 0.2–25 µg/mL |
HPLC determination of flutamide [18] | Stationary phase: nucleosil C18 column Mobile phase: acetonitrile: water (60:40, v/v). Flow rate: 1 mL/min Retention time: 6.35 min Linearity range: 0.0125–0.625 µg/mL |
Determination of flutamide in pharmaceutical formulation [19] | Stationary phase: Spheri-5 RP-18 column Mobile phase: acetonitrile: water (70: 30, v/v) Flow rate: 1 mL/min Detection wavelength: λex = 255 nm, λem = 375 nm Retention time: 3.9 min Linearity range: 0.1–0.6 µg/mL |
HPLC determination of flutamide [20] | Stationary phase: C8 column Mobile phase: methanol: acetonitrile: KH2PO4 (50 mM, pH = 3.2) (29:38:33, by volume) Flow rate: 1 mL/min Retention time: 2.9 min Linearity range: 0.0625–1.6 µg/mL |