Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods

BMC Chemistry

Table 6 Robustness and ruggedness studies of the developed method

HPTLC method
Robustness (SD)^a
Factor	Flu-1	Flutamide	Flu-3
1-Amount of acetic acid (± 0.01 mL/min)	0.03	0.03	0.03
2-% Tetrahydrofuran in the mobile phase (± 1%)	0.62	1.25	0.94
3-Detection wavelength (± 2 nm)	1.1	0.73	0.86
Ruggedness (SD)^a
1-Two analysts	0.009	0.004	0.009

HPLC method
Robustness (SD)^a
Factor	Flutamide	Flu-1	Flu-3
1-Mobile phase flow rate (± 0.05 mL/min)	2.916	0.742	0.446
2-% acetonitrile in the mobile phase (± 1%)	2.423	2.604	2.964
3-Detection wavelength (± 2 nm)	0.456	0.582	0.516
Ruggedness (SD)^a
1-Two analysts	0.196	0.412	0.269
2-Different acetonitrile manufacturer	0.939	1.33	0.287

ISSN: 2661-801X