From: Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods
HPTLC method | |||
---|---|---|---|
Robustness (SD)a | |||
Factor | Flu-1 | Flutamide | Flu-3 |
1-Amount of acetic acid (± 0.01 mL/min) | 0.03 | 0.03 | 0.03 |
2-% Tetrahydrofuran in the mobile phase (± 1%) | 0.62 | 1.25 | 0.94 |
3-Detection wavelength (± 2 nm) | 1.1 | 0.73 | 0.86 |
Ruggedness (SD)a | |||
1-Two analysts | 0.009 | 0.004 | 0.009 |
HPLC method | |||
---|---|---|---|
Robustness (SD)a | |||
Factor | Flutamide | Flu-1 | Flu-3 |
1-Mobile phase flow rate (± 0.05 mL/min) | 2.916 | 0.742 | 0.446 |
2-% acetonitrile in the mobile phase (± 1%) | 2.423 | 2.604 | 2.964 |
3-Detection wavelength (± 2 nm) | 0.456 | 0.582 | 0.516 |
Ruggedness (SD)a | |||
1-Two analysts | 0.196 | 0.412 | 0.269 |
2-Different acetonitrile manufacturer | 0.939 | 1.33 | 0.287 |