From: Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods
Parameters | HPTLC method | HPLC method | ||||
---|---|---|---|---|---|---|
Pure samples | Pure samples | |||||
Flutamide | Flu-1 | Flu-3 | Flutamide | Flu-1 | Flu-3 | |
Rangea | 0.1–3 | 0.3–2.5 | 0.3–3.5 | 2–50 | 1–50 | 5–200 |
Slope | − 645.07b 4902.40c | − 2070.03c 13121d | − 101.45c 825.60d | 43.6020 | 34.9780 | 6.4161 |
Intercept | 464.92 | 2938.80 | 1275.60 | − 17.9250 | − 5.1212 | 5.4658 |
Correlation (r) | 0.9998 | 0.9998 | 0.9999 | 0.9998 | 0.9999 | 0.9998 |
Accuracy Precision (SD)d | 99.98 | 100.99 | 100.67 | 99.86 | 99.45 | 99.57 |
Repeatabilitye, f | 1.12 | 0.35 | 2.47 | 0.863 | 1.224 | 1.016 |
Intermediateprecisione, g | 1.59 | 1.37 | 2.86 | 1.144 | 1.334 | 1.027 |
LODh | 0.03 | 0.09 | 0.09 | 0.45 | 0.31 | 1.65 |
LOQi | 0.09 | 0.28 | 0.27 | 1.35 | 0.93 | 4.95 |