Table 3 MST Stability data in RLMs matrix under different conditions
Nominal concentration (ng/mL) | Mean (ng/mL) | Standard deviation (SD) | Precision (RSD%) | Accuracy (RE%) |
|---|---|---|---|---|
Room temp. for 8 h | ||||
15 | 14.80 | 0.15 | 0.99 | − 1.34 |
50 | 48.16 | 0.20 | 0.41 | − 3.69 |
150 | 151.56 | 1.53 | 1.01 | 1.04 |
Three freeze–thaw cycles | ||||
15 | 14.62 | 0.07 | 0.50 | − 2.52 |
50 | 47.82 | 0.20 | 0.42 | − 4.36 |
150 | 151.82 | 0.72 | 0.47 | 1.22 |
Stored at 4 °C for 24 h | ||||
15 | 14.65 | 0.07 | 0.49 | − 2.32 |
50 | 47.02 | 0.50 | 1.07 | − 5.96 |
150 | 150.04 | 0.91 | 0.61 | 0.03 |
Stored at − 20 °C for 30 days | ||||
15 | 14.47 | 0.04 | 0.27 | − 3.55 |
50 | 46.55 | 0.19 | 0.42 | − 6.89 |
150 | 148.56 | 1.11 | 0.75 | − 0.96 |