Fig. 1From: Investigating and characterizing the binding activity of the immobilized calmodulin to calmodulin-dependent protein kinase I binding domain with atomic force microscopyEDTA titrations of ANS labeled CaM monitored by ANS fluorescence emission measurement. For purpose of comparison, all the fluorescence intensities were normalized to their respective 100% change. Sigmoidal fitting along with coefficient of determination (R 2) were also demonstrated in Fig. 1 Back to article page