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Table 1 Minimal inhibitory and bactericidal concentration and dehydrogenase activity of MeOH extract and its fractions from G. urbanum

From: Antimicrobial and antioxidant potential of different solvent extracts of the medicinal plant Geum urbanum L.

Test-bacteria

Parameters

Extracts/fractions

Control

MeOH

Petroleum ether

EtOAc

n-BuOH

Aerial parts

Roots

Aerial parts

Roots

Aerial parts

Roots

Aerial parts

Roots

S. aureus

NBIMCC 3359

MIC (mg/ml)

0.625

2.5

2.5**

2.5

0.078

0.156

0.156

1.25

Gentamicin (mg/L) 0.0312

DEHAa activity (%)

± STD

8.8 ± 2.2

20 ± 7.6

15 ± 1.0

15.8 ± 1.1

20.8 ± 2.2

47.5 ± 2.4

58.6 ± 3.4

47.9 ± 3.2

MBC (mg/ml)

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

S. aureus

ATCC 6538 P

MIC (mg/ml)

0.625

2.5

1.25

1.25

0.313

1.25

0.313

0.625

Gentamicin (mg/L) 0.125

DEHA activity (%)

± STD

2.6 ± 0.6

54.1 ± 2

14 ± 1.0

15 ± 1.0

21.5 ± 2.6

34.9 ± 2.7

41 ± 0.09

42.2 ± 5.7

MBC (mg/ml)

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

Methicillin resistant

S. aureus

NBIMCC 8327

MIC (mg/ml)

2.5

> 2.5

> 2.5

0.313

0.039

0.313

0.156

0.156

Gentamicin (mg/lL) 0.125

DEHA activity (%)

± STD

28.1 ± 0.1

4.2 ± 0.3

16.6 ± 1.1

6.9 ± 0.4

40.6 ± 2.0

37 ± 0.4

MBC (mg/ml)

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

S. epidermidis

NBIMCC 1093

MIC (mg/ml)

2.5

> 2.5

> 2.5

1.25

0.039

1.25

0.078

0.156

Gentamicin (mg/L) 0.0312

DEHA activity (%)

± STD

25.4 ± 1.9

15.23 ± 1.9

14.05 ± 2.7

47 ± 1.4

40.9 ± 7.1

38 ± 5.4

MBC (mg/ml)

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

> 2.5

B. cereus

ATCC 9634

MIC (mg/ml)

2.5

2.5

> 2.5

2.5

0.078

0.156

0.078

0.078

Gentamicin (mg/L) 0.125

DEHA activity (%)

± STD

31 ± 0.5

39.9 ± 1.8

30.3 ± 1.3

28.9 ± 0.05

26.2 ± 1.4

45.7 ± 2.8

51.4 ± 2.4

MBC (mg/ml)

> 2.5

> 2.5

> 2.5

> 2.5

0.625

0.625

> 2.5

> 2.5

DEHA activity (%)

± STD

0

1.9 ± 0.5

  1. ** 2.5 mg/ml is the higher test-concentration
  2. aDEHA (dehydrogenase) activity test refers to the enzyme NADH