Fig. 19
From: Ultra-high resolution X-ray structures of two forms of human recombinant insulin at 100 K
a Insugen (I) electron density in the vicinity of Zn2100 (Zn2) in molecule 1: an acetate molecule acetate 2101 has been modelled in this site close to HisB10 in chain B. Drawn with WinCoot 0.7 [19]. b The Zn site in molecule 1 of Insugen (I). Zn2 is coordinated to His B10 Nε in chain B as usually observed in insulin structures (e.g Baker et al. [1]) and unexpectedly to a highly ordered acetate molecule acetate2101. Drawn with Accelrys Discovery Studio 3 [23]. c Insugen (I) structure electron density showing the vicinity of Zn4100 (Zn1) and HisD10 in chain D. Unlike chain B there is no acetate in this site. Two water molecules have been located whose equivalents are not present in the vicinity of Zn2100 (Zn2) which has the substituted acetate2101. Drawn with WinCoot 0.7 [19]. d Intergen (II) electron density in the vicinity of Zn501 in molecule 1 B-chain. Both HisB10 Nε and water 617 coordinate Zn501. Water617 is the only coordinating water. There is no acetate molecule in this site. The same applies to site D. Drawn with WinCoot 0.7 [19]