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Table 2 The optimisation of the pH of phosphate buffer (buffer:methanol is 85:15)

From: A validated stability-indicating HPLC method for determination of brimonidine tartrate in BRI/PHEMA drug delivery systems

Mobile phase

Theoretical plates (N)

Symmetry

Retention time (tR) (min)

Peak shape

pH 2.5

9485

0.85

5.020

+

pH 2.8

8119

0.90

4.273

+++

pH 3.0

8218

0.93

4.270

+++

pH 3.5

8360

0.95

4.276

+++

pH 4.0

8076

0.90

4.262

+++

pH 5.0

7774

0.90

4.242

+++

pH 6.0

7551

0.90

4.241

+++

pH 7.0

7338

0.91

4.268

+++

pH 8.0

7178

0.88

7.861

+

  1. +, good peak shape