Mobile phase | Theoretical plates (N) | Symmetry | Retention time (tR) (min) | Peak shape |
---|---|---|---|---|
pH 2.5 | 9485 | 0.85 | 5.020 | + |
pH 2.8 | 8119 | 0.90 | 4.273 | +++ |
pH 3.0 | 8218 | 0.93 | 4.270 | +++ |
pH 3.5 | 8360 | 0.95 | 4.276 | +++ |
pH 4.0 | 8076 | 0.90 | 4.262 | +++ |
pH 5.0 | 7774 | 0.90 | 4.242 | +++ |
pH 6.0 | 7551 | 0.90 | 4.241 | +++ |
pH 7.0 | 7338 | 0.91 | 4.268 | +++ |
pH 8.0 | 7178 | 0.88 | 7.861 | + |