A validated stability-indicating HPLC method for determination of brimonidine tartrate in BRI/PHEMA drug delivery systems

Table 1 The optimisation of the mobil phases of solvent ratios with the Diamonsil C18 column

Mobile phase	Theoretical plates (N)	Symmetry	Peak shape
Methanol:water = 15:85	476	0.78	–
Acetonitrile:water = 15:85	607	0.70	–
0.010 M KH₂PO₄:water = 15:85	6483	0.63	–
0.025 M KH₂PO₄:water = 15:85	6258	0.61	–
0.050 M KH₂PO₄:water = 15:85	6616	0.66	–

ISSN: 2661-801X