Table 2 Design matrices of actual and predicted values of extraction temperatures (A), extraction times (B) and solvent ratios (water: ethanol) (C) for the extraction conditions of C. nutans leaves using the CCRD design
Run | Type | Independent variables | Code level | Response variable | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A (°C) | B (min) | C (v/v%) | A | B | C | Extraction yield (weight %) | DPPH radical scavenging activity (inhibition %) | Total phenolic content (mg GAE/g extract) | |||||
Act. | Pred. | Act. | Pred. | Act. | Pred. | ||||||||
1 | Factorial | 60 | 80 | 70:30 | −1 | −1 | −1 | 16.37 | 16.36 | 72.79 | 72.77 | 116.94 | 116.75 |
2 | Factorial | 80 | 80 | 70:30 | 1 | −1 | −1 | 24.50 | 24.48 | 69.87 | 69.86 | 121.63 | 121.99 |
3 | Factorial | 60 | 120 | 70:30 | −1 | 1 | −1 | 22.47 | 22.46 | 78.80 | 78.70 | 115.25 | 115.58 |
4 | Factorial | 80 | 120 | 70:30 | 1 | 1 | −1 | 17.23 | 17.26 | 54.43 | 54.52 | 99.50 | 102.08 |
5 | Factorial | 60 | 80 | 90:10 | −1 | −1 | 1 | 20.68 | 20.64 | 79.43 | 79.32 | 106.13 | 106.28 |
6 | Factorial | 80 | 80 | 90:10 | 1 | −1 | 1 | 23.26 | 22.02 | 69.45 | 69.54 | 103.00 | 102.83 |
7 | Factorial | 60 | 120 | 90:10 | −1 | 1 | 1 | 23.51 | 23.53 | 72.95 | 72.95 | 129.75 | 129.55 |
8 | Factorial | 80 | 120 | 90:10 | 1 | 1 | 1 | 23.18 | 11.60 | 75.77 | 41.90 | 107.00 | 107.35 |
9 | Axial | 53.18 | 100 | 80:20 | −1.68 | 0 | 0 | 22.15 | 22.16 | 74.41 | 74.54 | 111.00 | 111.02 |
10 | Axial | 86.82 | 100 | 80:20 | 1.68 | 0 | 0 | 18.97 | 18.96 | 46.08 | 45.98 | 97.00 | 96.76 |
11 | Axial | 70 | 66.36 | 80:20 | 0 | −1.68 | 0 | 18.25 | 18.29 | 80.22 | 80.24 | 117.00 | 116.98 |
12 | Axial | 70 | 133.64 | 80:20 | 0 | 1.68 | 0 | 14.69 | 14.65 | 57.94 | 61.98 | 120.00 | 119.80 |
13 | Axial | 70 | 100 | 63.18:36.82 | 0 | 0 | −1.68 | 17.23 | 23.02 | 74.70 | 74.72 | 119.50 | 119.27 |
14 | Axial | 70 | 100 | 96.82:3.18 | 0 | 0 | 1.68 | 21.85 | 21.86 | 69.60 | 69.61 | 72.25 | 114.90 |
15 | Center | 70 | 100 | 80:20 | 0 | 0 | 0 | 20.29 | 20.33 | 56.88 | 74.41 | 107.13 | 118.39 |
16 | Center | 70 | 100 | 80:20 | 0 | 0 | 0 | 21.29 | 20.33 | 74.35 | 74.41 | 117.69 | 118.39 |
17 | Center | 70 | 100 | 80:20 | 0 | 0 | 0 | 21.07 | 20.33 | 74.00 | 74.41 | 136.00 | 118.39 |
18 | Center | 70 | 100 | 80:20 | 0 | 0 | 0 | 22.44 | 20.33 | 73.53 | 74.41 | 102.75 | 118.39 |
19 | Center | 70 | 100 | 80:20 | 0 | 0 | 0 | 19.54 | 20.33 | 74.55 | 74.41 | 109.31 | 118.39 |
20 | Center | 70 | 100 | 80:20 | 0 | 0 | 0 | 20.37 | 20.33 | 74.34 | 74.41 | 119.06 | 118.39 |