Fig. 2

Morphological study of apoptosis in MCF-7 and MDA-MB231 induced by BTB: a by phase contrast microscopy (optical): (i, ii) untreated cells, (iii, iv) treated with 50 μg/ml and (v, vi) 100 μg/ml for 48 h. b Stained with Giemsa: (i, ii) untreated cells, (iii, iv) treated cells with 50 µg/ml, (v, vi) treated with 100 µg/ml for 48 h. c Stained with Hoechst 33342: (i, ii) treated with 50 µg/ml and (iii, iv) 100 µg/ml for 48 h. d Stained with propidium iodide: (i, ii) untreated cells, (iii, iv) treated with 50 µg/ml and (v, vi) 100 µg/ml for 48 h as shown by arrows