|
Run
|
Concentration of hexanola (mM × 10−4)
|
Enzyme amountb (%)
|
Reaction time (h)
|
Reaction temperature (°C)
|
Conversion (%)
|
Predicted (%)
|
Residual
|
|---|
|
X1
|
X2
|
X3
|
X4
|
Y
|
Y0
|
Y − Y0
|
|---|
|
12c
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
65.131
|
62.222
|
2.909
|
|
11
|
8.8 (+1)
|
35.0 (+1)
|
24.0 (−1)
|
60.0 (+1)
|
25.054
|
28.459
|
−3.404
|
|
4
|
2.2 (−1)
|
15.0 (−1)
|
24.0 (−1)
|
60.0 (+1)
|
17.151
|
23.714
|
−6.563
|
|
23
|
2.2 (−1)
|
15.0 (−1)
|
72.0 (+1)
|
40.0 (−1)
|
12.597
|
16.561
|
−3.964
|
|
5
|
2.2 (−1)
|
35.0 (+1)
|
24.0 (−1)
|
40.0 (−1)
|
18.375
|
9.751
|
8.623
|
|
26
|
8.8 (+1)
|
15.0 (−1)
|
72.0 (+1)
|
60.0 (+1)
|
29.213
|
36.068
|
−6.855
|
|
3
|
8.8 (+1)
|
35.0 (+1)
|
72.0 (+1)
|
40.0 (−1)
|
81.140
|
78.966
|
2.174
|
|
14c
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
64.554
|
62.222
|
2.332
|
|
1
|
2.2 (−1)
|
35.0 (+1)
|
72.0 (+1)
|
60.0 (+1)
|
19.431
|
24.580
|
−5.149
|
|
19
|
8.8 (+1)
|
15.0 (−1)
|
24.0 (−1)
|
40.0 (−1)
|
21.519
|
11.621
|
9.897
|
|
6
|
5.5 (0)
|
25.0 (0)
|
8.0 (−1.66)
|
50.0 (0)
|
17.720
|
25.369
|
−7.649
|
|
25
|
11.0 (1.66)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
35.044
|
34.562
|
0.482
|
|
13c
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
62.571
|
60.415
|
2.156
|
|
28c
|
5 (0)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
60.401
|
60.415
|
−0.014
|
|
22
|
5.5 (0)
|
8.3 (−1.66)
|
48.0 (0)
|
50.0 (0)
|
35.341
|
34.698
|
0.643
|
|
7
|
5.5 (0)
|
25.0 (0)
|
87.9 (1.66)
|
50.0 (0)
|
66.911
|
59.798
|
7.113
|
|
20
|
5.5 (0)
|
41.6 (1.66)
|
48.0 (0)
|
50.0 (0)
|
55.932
|
57.111
|
−1.179
|
|
15
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
66.6 (1.66)
|
45.865
|
41.460
|
4.405
|
|
27
|
0.0 (−1.66)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
0.000
|
1.018
|
−1.018
|
|
9
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
33.3 (−1.66)
|
38.220
|
43.160
|
−4.94
|
|
30
|
2.2 (−1)
|
35.0 (+1)
|
24.0 (−1)
|
60.0 (+1)
|
21.574
|
20.005
|
1.569
|
|
2
|
8.8 (+1)
|
15.0 (−1)
|
72.0 (+1)
|
40.0 (−1)
|
42.416
|
48.361
|
−5.945
|
|
18
|
8.8 (+1)
|
35.0 (+1)
|
72.0 (+1)
|
60.0 (+1)
|
69.420
|
65.198
|
4.222
|
|
10
|
8.8 (+1)
|
15.0 (−1)
|
24.0 (−1)
|
60.0 (+1)
|
15.065
|
13.174
|
1.891
|
|
29c
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
66.363
|
69.144
|
−2.781
|
|
16
|
2.2 (−1)
|
35.0 (+1)
|
72.0 (+1)
|
40.0 (−1)
|
24.885
|
28.172
|
−3.287
|
|
8
|
2.2 (−1)
|
15.0 (−1)
|
24.0 (−1)
|
40.0 (−1)
|
15.505
|
11.986
|
3.519
|
|
24
|
2.2 (−1)
|
15.0 (−1)
|
72.0 (+1)
|
60.0 (+1)
|
49.382
|
42.135
|
7.247
|
|
17
|
8.8 (+1)
|
35.0 (+1)
|
24.0 (−1)
|
40.0 (−1)
|
53.586
|
56.072
|
−2.486
|
|
21c
|
5.5 (0)
|
25.0 (0)
|
48.0 (0)
|
50.0 (0)
|
65.196
|
69.144
|
−3.948
|
-
aSubstrate molar ratio was selected correspondingly to 0.5 − 2(2.2–8.8 × 10−4 mM) hexanol to dihydrocaffeic acid (4.4 × 10−4 mM)
-
b15–35% relative to the total weight of substrates
-
c Center point
