Table 2 Central composite design: factor (Xi), response variable (Y) and residual
Run | Concentration of hexanola (mM × 10−4) | Enzyme amountb (%) | Reaction time (h) | Reaction temperature (°C) | Conversion (%) | Predicted (%) | Residual |
|---|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | Y | Y0 | Y − Y0 | |
12c | 5.5 (0) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 65.131 | 62.222 | 2.909 |
11 | 8.8 (+1) | 35.0 (+1) | 24.0 (−1) | 60.0 (+1) | 25.054 | 28.459 | −3.404 |
4 | 2.2 (−1) | 15.0 (−1) | 24.0 (−1) | 60.0 (+1) | 17.151 | 23.714 | −6.563 |
23 | 2.2 (−1) | 15.0 (−1) | 72.0 (+1) | 40.0 (−1) | 12.597 | 16.561 | −3.964 |
5 | 2.2 (−1) | 35.0 (+1) | 24.0 (−1) | 40.0 (−1) | 18.375 | 9.751 | 8.623 |
26 | 8.8 (+1) | 15.0 (−1) | 72.0 (+1) | 60.0 (+1) | 29.213 | 36.068 | −6.855 |
3 | 8.8 (+1) | 35.0 (+1) | 72.0 (+1) | 40.0 (−1) | 81.140 | 78.966 | 2.174 |
14c | 5.5 (0) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 64.554 | 62.222 | 2.332 |
1 | 2.2 (−1) | 35.0 (+1) | 72.0 (+1) | 60.0 (+1) | 19.431 | 24.580 | −5.149 |
19 | 8.8 (+1) | 15.0 (−1) | 24.0 (−1) | 40.0 (−1) | 21.519 | 11.621 | 9.897 |
6 | 5.5 (0) | 25.0 (0) | 8.0 (−1.66) | 50.0 (0) | 17.720 | 25.369 | −7.649 |
25 | 11.0 (1.66) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 35.044 | 34.562 | 0.482 |
13c | 5.5 (0) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 62.571 | 60.415 | 2.156 |
28c | 5 (0) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 60.401 | 60.415 | −0.014 |
22 | 5.5 (0) | 8.3 (−1.66) | 48.0 (0) | 50.0 (0) | 35.341 | 34.698 | 0.643 |
7 | 5.5 (0) | 25.0 (0) | 87.9 (1.66) | 50.0 (0) | 66.911 | 59.798 | 7.113 |
20 | 5.5 (0) | 41.6 (1.66) | 48.0 (0) | 50.0 (0) | 55.932 | 57.111 | −1.179 |
15 | 5.5 (0) | 25.0 (0) | 48.0 (0) | 66.6 (1.66) | 45.865 | 41.460 | 4.405 |
27 | 0.0 (−1.66) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 0.000 | 1.018 | −1.018 |
9 | 5.5 (0) | 25.0 (0) | 48.0 (0) | 33.3 (−1.66) | 38.220 | 43.160 | −4.94 |
30 | 2.2 (−1) | 35.0 (+1) | 24.0 (−1) | 60.0 (+1) | 21.574 | 20.005 | 1.569 |
2 | 8.8 (+1) | 15.0 (−1) | 72.0 (+1) | 40.0 (−1) | 42.416 | 48.361 | −5.945 |
18 | 8.8 (+1) | 35.0 (+1) | 72.0 (+1) | 60.0 (+1) | 69.420 | 65.198 | 4.222 |
10 | 8.8 (+1) | 15.0 (−1) | 24.0 (−1) | 60.0 (+1) | 15.065 | 13.174 | 1.891 |
29c | 5.5 (0) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 66.363 | 69.144 | −2.781 |
16 | 2.2 (−1) | 35.0 (+1) | 72.0 (+1) | 40.0 (−1) | 24.885 | 28.172 | −3.287 |
8 | 2.2 (−1) | 15.0 (−1) | 24.0 (−1) | 40.0 (−1) | 15.505 | 11.986 | 3.519 |
24 | 2.2 (−1) | 15.0 (−1) | 72.0 (+1) | 60.0 (+1) | 49.382 | 42.135 | 7.247 |
17 | 8.8 (+1) | 35.0 (+1) | 24.0 (−1) | 40.0 (−1) | 53.586 | 56.072 | −2.486 |
21c | 5.5 (0) | 25.0 (0) | 48.0 (0) | 50.0 (0) | 65.196 | 69.144 | −3.948 |