Different applications of isosbestic points, normalized spectra and dual wavelength as powerful tools for resolution of multicomponent mixtures with severely overlapping spectra

Table 4 Statistical comparison for the results obtained by the proposed spectrophotometric methods, the reported method [23] for the analysis of DRO and official methods [24, 25] for analysis of CAF and PCT

Values	DRO					CAF						PCT
	Proposed methods				Reported method [23]^a	Proposed methods					Official method [24]^b	Proposed methods						Official method [25]^c
	D⁰	RD-Iso	IRD	DD-RD-DW	Reported method [23]^a	AAS	AAM	S¹DD	RD-Iso	IRD	Official method [24]^b	AAS	AAM	S¹DD	RD-Iso	IRD	DD-RD-DW	Official method [25]^c
Mean	99.79	99.93	99.91	99.80	100.25	99.01	99.90	99.82	99.72	99.83	99.56	100.14	100.18	99.63	100.08	100.19	100.05	99.98
SD	0.48	0.57	0.30	0.49	0.39	0.81	0.78	0.65	0.79	0.70	0.59	0.21	0.58	0.40	0.45	0.16	0.27	0.25
RSD%	0.481	0.570	0.300	0.490	0.389	0.818	0.780	0.651	0.792	0.701	0.592	2.097	0.578	0.402	0.449	0.159	0.269	0.250
n	5	5	5	5	5	5	5	5	5	5	5	5	5	5	5	5	5	5
Variance	0.2304	0.324	0.0900	0.240	0.1521	0.6561	0.6084	0.4225	0.6241	0.49	0.3481	0.0441	0.3364	0.1600	0.2025	0.0256	0.0729	0.0625
Student’s t test^d (2.306)	1.663	1.036	0.1609	1.607		1.227	0.7774	0.6623	0.3629	0.5281		1.096	0.7081	1.659	0.4344	0.1523	0.4254
F value^d	1.515	2.136	1.690	1.579		1.885	1.748	1.214	1.793	1.408		1.417	5.382	2.560	3.240	2.441	1.166

^aDualwavelength spectrophotometric method at 271.5 and 280.0 nm
^bPotentiometric titration using 0.1 M perchloric acid
^cDirect UV spectrophotometric method, measuring the absorbance in water at 244 nm
^dThe values in the parenthesis are the corresponding theoretical values of t and F at P = 0.05

ISSN: 2661-801X