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Table 1 Assay parameters and method validation obtained by applying the proposed spectrophotometric methods for determination of DRO, CAF, PCT and PAP

From: Different applications of isosbestic points, normalized spectra and dual wavelength as powerful tools for resolution of multicomponent mixtures with severely overlapping spectra

Parameters DRO CAF
D0 RD-Iso IRD DD-RD-DW AAS AAM S1DD RD-Iso IRD
Linearity (µg/mL) 1–26 1.5–26
Slope 0.0722 1.4648 1.0018 0.3179 0.0525 0.9961 0.5027 2.7068 1.92333
Intercept 0.0036 0.0727 0.0036 0.0158 −0.0005 0.0091 −0.0008 −0.0043 −0.003
Correlation coefficient (r) 1.0000 1.0000 1.0000 0.9999 1.0000 1.0000 1.0000 1.0000 1.0000
Accuracy 99.74 ± 0.40 99.67 ± 0.41 100.04 ± 0.29 100.77 ± 0.37 100.05 ± 0.14 100.25 ± 0.02 99.99 ± 0.01 99.96 ± 0.02 100.08 ± 0.02
RSD%a 0.249 0.248 0.105 0.398 0.110 0.115 0.187 0.177 0.110
RSD%b 0.317 0.315 0.186 0.372 0.205 0.207 0.223 0.231 0.211
Parameters PCT PAP  
AAS AAM S1DD RD-Iso IRD DD-RD-DW D1 DD-RD-DW  
Linearity (µg/mL) 1–24 1–30        
Slope 0.0525 0.9961 0.9961 3.6059 1.9062 0.945 0.0213 1.8265  
Intercept 0.0005 0.0091 0.0091 0.0331 0.0175 0.0087 0.0010 0.0886  
Correlation coefficient (r) 1.0000 1.0000 1.0000 1.0000 1.0000 0.9999 0.9999 0.9999  
Accuracy 100.15 ± 0.31 100.30 ± 0.20 99.97 ± 0.25 100.01 ± 0.17 100.20 ± 0.21 99.98 ± 0.19 101.81 ± 0.40 100.146 ± 0.39  
RSD%a 0.380 0.315 0.382 0.279 0.201 0.374 0.727 0.732  
RSD%b 0.388 0.372 0.324 0.286 0.228 0.293 0.857 0.860  
  1. RSD%a, RSD%b: the intra-day, inter-day respectively (n = 3) relative standard deviation of concentrations DRO (6, 14, 22 µg/mL), CAF (6, 14, 22 µg/mL), PCT (6, 14, 22 µg/mL) and PAP (10, 18, 26 µg/mL)