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Table 1 Assay parameters and method validation obtained by applying the proposed spectrophotometric methods for determination of DRO, CAF, PCT and PAP

From: Different applications of isosbestic points, normalized spectra and dual wavelength as powerful tools for resolution of multicomponent mixtures with severely overlapping spectra

Parameters

DRO

CAF

D0

RD-Iso

IRD

DD-RD-DW

AAS

AAM

S1DD

RD-Iso

IRD

Linearity (µg/mL)

1–26

1.5–26

Slope

0.0722

1.4648

1.0018

0.3179

0.0525

0.9961

0.5027

2.7068

1.92333

Intercept

0.0036

0.0727

0.0036

0.0158

−0.0005

0.0091

−0.0008

−0.0043

−0.003

Correlation coefficient (r)

1.0000

1.0000

1.0000

0.9999

1.0000

1.0000

1.0000

1.0000

1.0000

Accuracy

99.74 ± 0.40

99.67 ± 0.41

100.04 ± 0.29

100.77 ± 0.37

100.05 ± 0.14

100.25 ± 0.02

99.99 ± 0.01

99.96 ± 0.02

100.08 ± 0.02

RSD%a

0.249

0.248

0.105

0.398

0.110

0.115

0.187

0.177

0.110

RSD%b

0.317

0.315

0.186

0.372

0.205

0.207

0.223

0.231

0.211

Parameters

PCT

PAP

 

AAS

AAM

S1DD

RD-Iso

IRD

DD-RD-DW

D1

DD-RD-DW

 

Linearity (µg/mL)

1–24

1–30

       

Slope

0.0525

0.9961

0.9961

3.6059

1.9062

0.945

0.0213

1.8265

 

Intercept

0.0005

0.0091

0.0091

0.0331

0.0175

0.0087

0.0010

0.0886

 

Correlation coefficient (r)

1.0000

1.0000

1.0000

1.0000

1.0000

0.9999

0.9999

0.9999

 

Accuracy

100.15 ± 0.31

100.30 ± 0.20

99.97 ± 0.25

100.01 ± 0.17

100.20 ± 0.21

99.98 ± 0.19

101.81 ± 0.40

100.146 ± 0.39

 

RSD%a

0.380

0.315

0.382

0.279

0.201

0.374

0.727

0.732

 

RSD%b

0.388

0.372

0.324

0.286

0.228

0.293

0.857

0.860

 
  1. RSD%a, RSD%b: the intra-day, inter-day respectively (n = 3) relative standard deviation of concentrations DRO (6, 14, 22 µg/mL), CAF (6, 14, 22 µg/mL), PCT (6, 14, 22 µg/mL) and PAP (10, 18, 26 µg/mL)