Table 2 Robustness data for the proposed method
Variation | Resolution | Theoretical plates | Assay (%) | ||
|---|---|---|---|---|---|
IND | GLY | IND | GLY | ||
28% ACN | 4.81 | 4785 | 4658 | 99.86 | 100.23 |
32% ACN | 3.57 | 4924 | 4826 | 100.71 | 100.96 |
pH 3.3 | 4.13 | 4875 | 4732 | 100.49 | 100.91 |
pH 3.7 | 4.32 | 4817 | 4715 | 100.37 | 100.35 |
Flow (1.9 mL) | 4.42 | 4858 | 4715 | 100.35 | 100.34 |
Flow (2.1 mL) | 3.91 | 4894 | 4737 | 100.52 | 100.87 |
Wavelength 208 (nm) | – | 4889 | 4734 | 100.73 | 101.14 |
Wavelength 212 (nm) | – | 4862 | 4719 | 100.29 | 100.12 |
Temperature 33 °C | 4.33 | 4861 | 4712 | 100.3 | 100.24 |
Temperature 37 °C | 4.14 | 4884 | 4741 | 100.61 | 100.81 |
Without variation | 4.25 | 4870 | 4725 | 100.43 | 100.62 |