Table 2 The Box–Behnken design and the yield of Ramaria botrytis polysaccharide
Run | X 1/water to raw material ratio (mL g−1) | X 2/extraction temperature (°C) | X 3/extraction time (h) | Extraction yield (%) | Predicted yield (%) |
|---|---|---|---|---|---|
1 | 10 (−1) | 90 (1) | 1.5 (0) | 9.10 | 9.11 |
2 | 20 (1) | 70 (−1) | 1.5 | 6.47 | 6.46 |
3 | 20 | 80 (0) | 2.0 (1) | 7.97 | 7.82 |
4 | 15 (0) | 80 | 1.5 | 8.57 | 8.81 |
5 | 15 | 80 | 1.5 | 8.45 | 8.81 |
6 | 15 | 70 | 1.0 (−1) | 6.50 | 6.25 |
7 | 20 | 90 | 1.5 | 9.90 | 9.80 |
8 | 15 | 80 | 1.5 | 8.87 | 8.81 |
9 | 15 | 70 | 2.0 | 6.20 | 6.36 |
10 | 15 | 80 | 1.5 | 9.07 | 8.81 |
11 | 15 | 90 | 2.0 | 9.60 | 9.85 |
12 | 10 | 80 | 1.0 | 6.70 | 6.86 |
13 | 10 | 80 | 2.0 | 7.28 | 7.02 |
14 | 10 | 70 | 1.5 | 5.97 | 6.07 |
15 | 15 | 90 | 1.0 | 9.30 | 9.14 |
16 | 20 | 80 | 1.0 | 6.90 | 7.16 |
17 | 15 | 80 | 1.5 | 9.07 | 8.81 |