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Table 2 The Box–Behnken design and the yield of Ramaria botrytis polysaccharide

From: Extraction, purification, characterization and antioxidant activities of polysaccharides from Ramaria botrytis (Pers.) Ricken

Run X 1/water to raw material ratio (mL g−1) X 2/extraction temperature (°C) X 3/extraction time (h) Extraction yield (%) Predicted yield (%)
1 10 (−1) 90 (1) 1.5 (0) 9.10 9.11
2 20 (1) 70 (−1) 1.5 6.47 6.46
3 20 80 (0) 2.0 (1) 7.97 7.82
4 15 (0) 80 1.5 8.57 8.81
5 15 80 1.5 8.45 8.81
6 15 70 1.0 (−1) 6.50 6.25
7 20 90 1.5 9.90 9.80
8 15 80 1.5 8.87 8.81
9 15 70 2.0 6.20 6.36
10 15 80 1.5 9.07 8.81
11 15 90 2.0 9.60 9.85
12 10 80 1.0 6.70 6.86
13 10 80 2.0 7.28 7.02
14 10 70 1.5 5.97 6.07
15 15 90 1.0 9.30 9.14
16 20 80 1.0 6.90 7.16
17 15 80 1.5 9.07 8.81