Simultaneous determination of newly developed antiviral agents in pharmaceutical formulations by HPLC-DAD

BMC Chemistry

Table 4 Robustness of the proposed HPLC method

Parameter	PAR		DAS		RIT		OMB
Parameter	RSD % of peak areas	k ± SD	RSD % of peak areas	k ± SD	RSD % of peak areas	k ± SD	RSD % of peak areas	k ± SD
Percentage of acetonitrile in the mobile phase [64, 65 and 66 ml]	0.290	0.48 ± 0.007	0.177	2.08 ± 0.005	0.446	2.81 ± 0.001	0.335	6.11 ± 0.002
pH of the aqueous phase [6.9, 7 and 7.1]	0.363	0.53 ± 0.011	0.178	2.03 ± 0.010	0.413	2.73 ± 0.001	0.471	5.85 ± 0.003
Flow rate [0.9, 1, and 1.1 ml/min]	0.239	0.53 ± 0.005	0.182	2.12 ± 0.005	0.32	2.87 ± 0.000	0.579	6.25 ± 0.003
Injection volume [15, 20, 25 µl]	0.255	0.53 ± 0.024	0.141	1.97 ± 0.026	0.80	2.68 ± 0.01	0.314	5.66 ± 0.007
Detection wavelength [253, 254 and 255 nm]	0.403	0.55 ± 0.007	0.089	1.99 ± 0.003	0.394	2.69 ± 0.001	0.580	5.75 ± 0.002

ISSN: 2661-801X