Table 2 Regression and statistical parameters for the determination of DAAs using the proposed HPLC method

Linearity range (µg/ml)

2.5–60

1.25–30

1.7–40

0.42–10

LOD (µg/ml)^a

0.0024

0.00488

0.0521

0.0065

LOQ (µg/ml)^b

0.0049

0.0098

0.1042

0.0130

Intercept

0.0940

0.0525

0.0057

0.0124

Slope

0.1233

0.2122

0.0144

0.1725

Correlation coefficient (r)

0.9995

0.9998

0.9996

0.9997

S ^c_a

0.0415

0.0230

0.0031

0.0137

S ^d_b

0.0013

0.0015

0.0002

0.0084

S ^e_y/x

0.0682

0.0378

0.0051

0.0016

F^f

8441.8243

20320.4610

9008.7822

11307.0622

Significance F

8.4127E−08

1.4525E−08

7.3875E−08

4.6902E−08