Fig. 19

Schematic illustration of the free-energy barrier to nucleation as a function of crystal size, a micrograph image of a docked droplet, b the reservoir is filled with 6 M NaCl solution causing water to transfer from the droplet into the reservoir causing precipitation of poor quality nanocrystallites, c the reservoir is filled with 2 M NaCl solution resulting in some re-dissolution of protein and subsequent nucleation of a single crystal. Reprinted with permission from Ref. [34]. Copyright 2007 American Chemical Society