Rapid micellar HPLC analysis of loratadine and its major metabolite desloratadine in nano-concentration range using monolithic column and fluorometric detection: application to pharmaceuticals and biological fluids

Table 6 Assay results for the determination of the studied analytes in spiked human urine, human plasma and breast milk using the proposed method

Matrix	Parameters	LOR			DSL
Matrix	Parameters	Amount taken (ng/mL)	Amount found (ng/mL)	Relative recovery (%)^a	Amount taken (ng/mL)	Amount found (ng/mL)	Relative recovery (%)^a
Urine	Data	5.0	4.744	94.88	5.0	4.657	93.14
		20.0	20.370	101.85	20.0	20.520	102.60
		50.0	49.800	99.97	50.0	49.800	99.68
	Mean			98.81			98.47
	SD			3.57			4.84
	% RSD			3.61			4.92
	% error			2.08			2.84
Plasma	Data	5.0	4.687	93.74	5.0	4.584	91.68
		20.0	20.477	102.39	20.0	20.631	103.16
		50.0	49.900	99.71	50.0	49.800	99.61
	Mean			98.61			98.15
	SD			4.43			5.88
	% RSD			4.49			5.99
	% error			2.59			3.46
Breast milk	Data	5.0	4.700	94.0	5.0	4.624	92.48
		20.0	20.457	102.29	20.0	20.566	102.83
		50.0	49.900	99.72	50.0	49.800	99.63
	Mean			98.67			98.31
	SD			4.24			5.3
	% RSD			4.3			5.39
	% error			2.48			3.11