BMC Chemistry

Table 4 Assay results for the determination of the studied analytes in pure form by the proposed and official method [43]

From: Rapid micellar HPLC analysis of loratadine and its major metabolite desloratadine in nano-concentration range using monolithic column and fluorometric detection: application to pharmaceuticals and biological fluids

Analyte	Proposed method			Official method [43]
Analyte	Amount taken (ng/mL)	Amount found (ng/mL)	% recovery^a	Amount taken (µg/mL)	Amount found (µg/mL)	% recovery^a
LOR	20.0	20.400	102.01	5.0	4.900	98.01
	50.0	50.300	100.61	30.0	30.030	100.1
	80.0	79.700	99.63	50.0	49.990	99.98
	100.0	99.200	99.21
	200.0	200.400	100.21
Mean %			100.33			99.37
±SD			1.08			1.18
t test^b	1.196 (2.447)
F test^b	1.182 (6.944)
DSL	20.0	20.439	102.20	5.0	4.940	98.71
	50.0	49.684	99.37	30.0	30.260	100.87
	80.0	79.456	98.32	50.0	49.720	99.44
	100.0	100.400	100.35
	200.0	200.100	100.05
Mean %			100.26			99.67
±SD			1.17			1.10
t test^b	0.697 (2.447)
F test^b	1.137 (19.247)

^aEach result is the average of three separate determinations
^bThe values between parentheses are the tabulated t and F values at P = 0.05

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