TY - JOUR AU - Wei, Jinfeng AU - Cao, Pengran AU - Wang, Jinmei AU - Kang, Wenyi PY - 2016 DA - 2016/11/29 TI - Analysis of tilianin and acacetin in Agastache rugosa by high-performance liquid chromatography with ionic liquids-ultrasound based extraction JO - Chemistry Central Journal SP - 76 VL - 10 IS - 1 AB - Ionic liquid 1-butyl-3-methylimidazolium bromide-methanol-based ultrasonic-assisted extraction (ILUAE) was used to extract tilianin and acacetin from the aerial parts of Agastache rugose (A. rugose), and simultaneously determined by reversed phase high performance liquid chromatographic (RP-HPLC) method with ultraviolet detection (RP-HPLC-UV). An InertSustain RP-C18 column was used with the mobile phase consisting of methanol and 0.2% acetic acid as gradient elution at the detection wavelength of 332 nm. The flow rate was 0.8 mL/min, and the column temperature was 30 °C. Under the optimized conditions, tilianin and acacetin displayed good linearity in the ranges of 0.0595–4.76 and 0.0585–4.68 μg/mL, respectively, with the average recoveries being 96.93 and 97.88%, respectively. The method of ILUAE was compared with the traditional methods, it exhibited higher efficiency, higher reproducibility and environmental friendly in analyzing the active compounds in traditional Chinese medicines (TCMs). SN - 1752-153X UR - https://doi.org/10.1186/s13065-016-0223-7 DO - 10.1186/s13065-016-0223-7 ID - Wei2016 ER -