Table 1 The sequences of the primers and probes for the qPCR and dPCR analysis of 3 plasmids
primer/probe | Sequence 5′–3′ | Amplicon size (bp) |
|---|---|---|
fliC-FP | CCGCGAGCGAAGGTAGTG | 75 |
fliC-RP | CAGGAGTTGCTTTTGCGATAGTATAT | |
fliC-probe* | FAM-CGGTGCTTCTCTGACATTCAATGGCA- TAMRA | |
stx1-FP | TGCAGATAAATCGCCATTCG | 123 |
stx1-RP | AAGCTTCAGCTGTCACAGTAACAAA | |
stx1-probe | FAM-ACCTCACTGACGCAGTCTGTGGCAAGA- TAMRA | |
stx2-FP | CACTGTCTGAAACTGCTCCTGTTT | 78 |
stx2-RP | TGCTGATTCTCCCCCAGTTC | |
stx2-probe | FAM-CGGTGCTTCTCTGACATTCAATGGCA- TAMRA |