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Fig. 6 | Chemistry Central Journal

Fig. 6

From: Quantification of plasmid DNA reference materials for Shiga toxin-producing Escherichia coli based on UV, HR-ICP-MS and digital PCR

Fig. 6

dPCR results on microarrray chips of 3 plasmids: pFliC, pStx1, pStx2 (from top to bottom). 3072 chambers on one chip were divided into 4 groups (separated by the red dashed lines) for 4 diluted samples (S1, S2, S3, S4). The green points and the black points represented the positive signals and the negative signals respectively, and the gray points were ineffective reactions due to either suspiciously abnormal baseline fluorescence or low fluorescence scores. The X marks were empty well mainly due to failed sample uploading. The down left squares of all the 4 groups were negative controls. Column diagrams on the right showed the quantification results of 4 dilutions

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