Tissue-specific metabolite profiling and quantitative analysis of ginsenosides in Panax quinquefolium using laser microdissection and liquid chromatography–quadrupole/time of flight-mass spectrometry

Chen, Yujie; Xu, Liang; Zhao, Yuancen; Zhao, Zhongzhen; Chen, Hubiao; Yi, Tao; Qin, Minjian; Liang, Zhitao

doi:10.1186/s13065-015-0141-0

Table 3 Contents of ginsenosides in the tissues from Panax quinquefolium samples

From: Tissue-specific metabolite profiling and quantitative analysis of ginsenosides in Panax quinquefolium using laser microdissection and liquid chromatography–quadrupole/time of flight-mass spectrometry

Sample no.	Tissue	Amount in unit area (ng/10⁵μm²)
Sample no.	Tissue	Rg ^a₁	Re	Rh₁	Rg₂	Rb₁	Rb₂	Rd	Sum
Pq1 main root	Cork	67.31	34.58	0.40	8.83	13,247.66	25.51	13.18	13,397.47
	Cortex	18.77	9.50	–^b	1.83	5576.43	1.02	1.05	5608.60
	Phloem	11.53	7.46	0.25	1.87	4734.50	1.28	1.53	4758.42
	Secondary xylem	9.38	10.74	–	2.11	3176.85	2.20	1.69	3202.97
	Primary xylem	31.12	12.11	0.30	2.07	8104.59	2.15	1.49	8153.83
Pq1 branch root	Cork	74.68	50.67	–	1.16	16,897.70	31.86	7.00	17,063.07
	Cortex	10.84	9.88	–	2.81	7608.60	1.63	3.24	7637.00
	Phloem	5.50	5.46	0.35	2.17	5073.24	0.56	2.72	5090.00
	Xylem	8.10	9.36	–	3.97	7239.49	1.68	12.29	7274.89
Pq2 main root	Cork	130.53	69.38	0.27	3.45	16,012.69	42.06	27.09	16,285.47
	Cortex	36.33	19.63	–	0.75	5244.41	1.16	0.95	5303.23
	Phloem	16.39	8.66	–	2.57	3840.21	1.17	0.72	3869.72
	Secondary xylem	23.34	28.46	–	7.57	2344.29	2.51	1.50	2407.67
	Primary xylem	27.65	29.74	–	4.72	2688.51	3.21	0.93	2754.76
Pq2 branch root	Cork	62.44	46.66	0.30	13.93	17,558.77	52.02	29.52	17,763.64
	Cortex	11.64	8.78	0.36	2.84	3371.72	1.44	2.04	3398.82
	Phloem	11.57	8.17	0.37	4.20	3159.24	1.82	3.55	3188.92
	Xylem	23.09	18.41	0.34	9.31	5805.28	1.48	17.17	5875.08
Pq3 main root	Cork	41.66	18.92	0.39	4.15	269,855	16.80	7.24	269,944.16
	Cortex	18.67	7.19	0.59	1.61	145,606.6	3.61	1.15	145,639.42
	Phloem	11.31	6.40	0.51	0.98	67,598.38	–	1.21	67,618.79
	Secondary xylem	11.69	6.42	0.31	0.84	50,655.09	–	–	50,674.35
	Primary xylem	10.03	5.35	0.33	0.87	19,113.26	0.46	0.30	19,130.60
Pq3 branch root	Cork	23.16	20.68	0.32	4.98	252,865.9	12.32	17.92	252,945.28
	Cortex	6.69	6.20	0.39	2.06	114,430.5	3.50	4.44	114,453.78
	Phloem	5.17	4.43	0.32	1.56	85,663.43	0.95	3.07	85,678.93
	Xylem	6.18	5.95	0.27	0.30	134,882.3	0.70	9.10	134,904.80
Pq4 main root	Cork	48.13	23.62	0.32	0.56	11,972.59	20.01	8.35	12,073.58
	Cortex	11.50	5.10	0.31	0.84	4151.39	1.61	1.43	4172.18
	Phloem	11.85	5.45	0.33	0.52	1685.59	–	1.28	1705.02
	Secondary xylem	9.70	5.35	0.48	0.69	2659.33	1.51	1.45	2678.51
	Primary xylem	6.37	3.43	0.43	0.54	1766.77	0.74	0.47	1778.75
Pq4 branch root	Cork	19.34	20.25	0.30	3.61	20,298.81	19.70	23.67	20,385.68
	Cortex	7.09	7.63	0.40	1.48	12,388.83	5.48	7.41	12,418.32
	Phloem	2.94	4.66	0.32	1.07	5156.83	1.73	9.49	5177.04
	Xylem	7.50	8.57	0.35	2.25	15,479.39	2.95	9.38	15,510.39
Pq5	Cork	1723.58	838.53	10.24	11.41	869.15	167.96	229.08	3849.94
	Cortex	920.69	365.92	4.64	3.35	764.67	11.79	20.06	2091.13
	Phloem	527.99	390.62	2.07	1.50	885.82	6.30	70.71	1884.99
	Secondary xylem	798.60	434.04	0.95	2.28	821.14	6.09	26.04	2089.14
	Primary xylem	1028.47	924.56	1.19	5.86	1365.07	32.65	144.22	3502.02
Pq6	Cork	670.07	34.99	6.03	1.00	582.31	149.25	155.58	1599.24
	Cortex	320.81	13.99	3.79	0.84	364.01	41.22	47.94	792.61
	Phloem	417.83	18.60	2.80	0.94	432.25	5.95	25.73	904.10
	Cambium	605.12	26.43	7.10	1.03	600.16	6.08	40.85	1286.77
	Secondary xylem	906.45	35.99	4.26	0.85	814.07	7.77	59.66	1829.04
	Primary xylem	1501.30	74.73	5.11	1.32	1115.92	23.22	179.22	2900.82
Pq7	Cork	166.40	327.34	1.71	3.93	401.22	66.79	127.19	1094.58
	Cortex	174.18	207.77	1.30	2.98	163.75	16.12	24.81	590.91
	Phloem	119.12	131.49	0.65	1.40	191.36	4.16	21.26	469.43
	Secondary xylem	158.80	110.53	0.60	0.88	157.42	4.33	3.80	436.35
	Primary xylem	187.65	173.03	0.71	0.92	333.41	11.19	30.31	737.22
Pq8	Cork	149.28	1827.33	0.67	12.70	1347.97	41.12	429.50	3808.57
	Cortex	180.35	714.05	0.74	19.35	1173.10	80.68	82.68	2250.96
	Phloem	141.83	732.05	0.56	6.91	1002.23	9.38	49.03	1941.99
	Cambium	144.34	723.85	0.62	9.33	1154.96	5.40	61.17	2099.69
	Secondary xylem	144.52	987.34	0.80	9.24	1478.13	11.02	163.51	2794.55
	Primary xylem	145.17	1302.97	0.95	11.91	1365.79	12.07	218.33	3057.19
Pq9	Cork	907.61	14.06	2.08	0.88	799.16	195.43	170.42	2089.63
	Cortex	160.10	1.99	–	–	179.45	7.47	4.61	353.61
	Phloem	74.54	1.52	–	0.95	60.01	4.97	2.16	144.15
	Secondary xylem	392.43	2.41	–	–	430.97	3.61	22.49	851.91
	Primary xylem	676.25	2.78	0.84	–	1019.24	5.45	69.53	1774.09
Pq10	Cork	668.61	712.57	0.84	6.36	986.29	19.83	67.40	2461.89
	Cortex	139.10	669.75	0.54	14.92	635.65	78.61	39.19	1577.77
	Phloem	123.70	611.79	0.61	6.61	434.33	3.12	14.90	1195.07
	Secondary xylem	146.61	697.48	0.66	5.26	538.81	16.77	19.34	1424.93
	Primary xylem	147.68	743.10	0.62	7.22	714.65	2.12	20.17	1635.56
Pq11	Cork	62.97	537.33	0.88	5.07	511.33	65.37	188.27	1371.23
	Cortex	24.68	320.33	–	3.50	503.92	2.51	36.75	891.69
	Phloem	21.42	344.88	–	3.88	670.87	2.04	83.75	1126.83
	Secondary xylem	8.58	340.35	–	3.60	564.94	2.98	159.17	1079.62
	Primary xylem	22.13	619.29	0.56	7.31	916.71	3.81	364.94	1934.75
Pq12	Cork	115.07	518.18	1.85	6.69	634.75	104.45	319.73	1700.73
	Cortex	67.05	342.52	0.55	3.34	560.80	9.01	43.48	1026.75
	Phloem	69.79	375.80	–	3.56	871.87	3.74	48.98	1373.75
	Secondary xylem	45.97	610.82	0.61	4.39	1117.76	6.46	211.26	1997.28
	Primary xylem	147.28	871.49	0.47	5.95	1021.44	9.45	132.19	2188.28
Pq13	Cork	82.82	766.07	0.92	22.68	568.79	88.17	125.20	1654.66
	Cortex	33.11	428.44	–	6.46	453.02	7.45	37.26	965.74
	Phloem	34.04	547.62	–	5.32	526.29	3.31	41.08	1157.65
	Secondary xylem	41.02	453.95	0.52	8.36	772.36	5.45	104.84	1386.50
	Primary xylem	37.32	893.53	–	14.27	922.80	3.81	166.35	2038.07

^aGinsenoside
^bUnder detection limit

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ISSN: 2661-801X

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