Table 2 Purification summary of MnP produced by G. lucidum IBL-05 in solid state fermentation
Purification Steps | Total volume (mL) | Enzyme activity (U/mL) | Protein contents (mg/mL) | Specific activity (U/mg) | Purification fold |
|---|---|---|---|---|---|
Crude extract | 300 | 717.4 | 4.34 | 132.32 | 1 |
Ammonium Sulphate ppt. | 22 | 613.7 | 2.45 | 273.01 | 1.73 |
Dialysis | 21 | 598.09 | 2.13 | 390.91 | 1.95 |
DEAE-cellulose | 12 | 584.3 | 1.68 | 420.34 | 2.78 |
Sephadex G-100 | 9 | 569.6 | 0.9 | 539.59 | 3.43 |