Figure 3

Effect of the amount of magnetic beads on the sensitivity. Here, 22.7 ng/mL (ie. 0.005 μg/well) of IgG with varied amounts of anti-IgG-labeled MPs were incubated for 20 min and washed twice with PBS containing 0.1% BSA. IgG was then reacted with anti-IgG-HRP for 30 min and washed twice with PBS containing 0.1% triton. Furthermore, 200 μL of PBS containing TMB/H2O2 was added, and 150-μL aliquots were transferred to another microplate after 30 min. Finally, 50 μL of 1 M HCl was added to this microplate, and its absorbance was measured at 450 nm.