Figure 2

Optimization of immunoreaction time. (a) Effect of reaction time of anti-IgG-labeled MPs and IgG. (b) Effect of reaction time of anti-IgG-HRP with the microparticles to form the sandwich. (c) Relationship between washing times and the cleanliness of unreacted anti-IgG-HRP in the solution. “Unreactive” represents aliquots from the suspension of the mixture of anti-IgG-HRP and MP-anti-IgG-IgG after incubation. “1st washed” represents aliquots from the solution of the first washing. “2nd washed” represents aliquots from the solution after the second washing. “Blank” represents aliquots that contained only TMB/H₂O₂ in PBS.