Table 1 The control program of AuNPs- luminol- potassium ferricyanide SIA-chemiluminescence detection of NLX

‘Loop Start (♯) 1

Next sample

Counter clockwise

Peristaltic pump

Delay (s)

% 50

Peristaltic pump off

25

Detector

ON

Syringe pump

Valve position IN

Syringe pump

Set flow rate (μL s ⁻¹ )

100

Syringe pump

Aspirate (μL)

1500

Pump filled with carrier

Syringe pump

Delay until done

Multiposition valve

Set valve position

3

Syringe pump

Set flow rate (μL s ⁻¹ )

100

Syringe pump

Aspirate (μL)

50

(Luminol 5.0×10 ⁻⁴ mol L ⁻¹ )

Multiposition valve

Set valve position

2

Syringe pump

Set flow rate (μL s ⁻¹ )

100

Syringe pump

Aspirate (μL)

50

(Gold nanoparticles 1.0×10 ⁻⁵ mol L ⁻¹ )

Multiposition valve

Set valve position

5

Syringe pump

Set flow rate (μL s ⁻¹ )

100

Syringe pump

Aspirate (μL)

30

Sample (NLX)

Syringe pump

Delay until done

Multiposition valve

Set valve position

4

Syringe pump

Set flow rate (μL s ⁻¹ )

100

Syringe pump

Aspirate (μL)

30

(Potassium ferricyanide1.0×10 ⁻² mol L ⁻¹ )

Syringe pump

Delay until done

Multiposition valve

Detector

7

Syringe pump

Set flow rate (μL s ⁻¹ )

100

PMT

Start scan

Syringe pump

Empty

Syringe pump

Delay until done

PMT

Stop scans

Refresh plat

Loop end