Table 2 Summary of assay validation results
Drugs in (pg/mg) | Conctrn. QC's | Linear range | LOD | r 2 | Inra-day (n = 6) | Inter-day(n = 6) | ||
|---|---|---|---|---|---|---|---|---|
Precision, % CV | Accuracy,% | Precision, % CV | Accuracy,% | |||||
Nandrolone | 10 | 0.1-100 | 0.05 | 0.9998 | 3.23 | 100.1 | 4.82 | 99.8 |
25 | 1.96 | 100.4 | 1.95 | 99.4 | ||||
50 | 1.41 | 99.9 | 1.39 | 100.2 | ||||
Stanozolol | 10 | 0.1-100 | 0.05 | 0.9995 | 6.3 | 102.1 | 9.4 | 101.2 |
25 | 10.2 | 98.5 | 7.1 | 99.8 | ||||
50 | 8.6 | 99.4 | 3.2 | 101.3 | ||||
Boldenone | 10 | 0.5-100 | 0.25 | 0.9998 | 4.9 | 99.9 | 3.4 | 101.1 |
25 | 3.3 | 100.3 | 2.7 | 99.8 | ||||
50 | 2.5 | 99.7 | 2.3 | 98.7 | ||||
Cocaine | 10 | 0.5-100 | 0.25 | 0.9998 | 3.5 | 98.9 | 3.2 | 99.8 |
25 | 4.3 | 99.4 | 3.3 | 98.9 | ||||
50 | 2.9 | 99.9 | 2.3 | 97.8 | ||||
Benzoylecgonine | 10 | 0.5-100 | 0.25 | 0.9987 | 3.5 | 107.2 | 4.7 | 100.5 |
25 | 2.1 | 102.3 | 10.9 | 102.6 | ||||
50 | 3.3 | 100.1 | 8.9 | 99.9 | ||||
Amphetamine | 10 | 0.25-100 | 0.1 | 0.9979 | 7.1 | 98.8 | 3.2 | 100.5 |
25 | 5.9 | 99.6 | 2.7 | 100.1 | ||||
50 | 3.2 | 89.9 | 3.2 | 100.3 | ||||
Methamphetamine | 10 | 0.5-100 | 0.25 | 0.9988 | 5.2 | 98.3 | 5.3 | 100.2 |
25 | 2.3 | 102.6 | 2.1 | 103.3 | ||||
50 | 4.1 | 100.9 | 3.6 | 108.1 | ||||
Ephedrine | 10 | 1-100 | 0.5 | 0.9945 | 3.8 | 99.3 | 4.3 | 101.2 |
25 | 3.5 | 98.7 | 2.1 | 99.8 | ||||
50 | 3.2 | 101.2 | 3.2 | 99.9 | ||||
MDMA | 10 | 0.5-100 | 0.25 | 0.9968 | 5.1 | 99.4 | 5.1 | 100.3 |
25 | 2.4 | 103.2 | 3.3 | 110.2 | ||||
50 | 3.7 | 101.6 | 6.2 | 104.2 | ||||
THC | 10 | 1-100 | 0.5 | 0.9996 | 2.1 | 107.3 | 4.3 | 109.2 |
25 | 3.2 | 110.2 | 2.6 | 105.3 | ||||
50 | 5.1 | 101.3 | 3.9 | 103.2 | ||||
THC-COOH | 10 | 1-100 | 0.5 | 0.9992 | 4.3 | 100.1 | 3.3 | 101.1 |
25 | 3.6 | 98.9 | 3.9 | 99.9 | ||||
50 | 2.9 | 98.6 | 4.3 | 98.9 | ||||
11-OH-THC | 10 | 0.5-100 | 0.25 | 0.9998 | 3.6 | 89.8 | 5.3 | 90.5 |
25 | 2.1 | 89.9 | 5.9 | 89.6 | ||||
50 | 5.3 | 90.9 | 2.1 | 99.8 | ||||
Morphine | 10 | 0.5-100 | 0.25 | 0.999 | 4.2 | 89.3 | 3.2 | 90.9 |
25 | 6.3 | 88.9 | 5.3 | 91.8 | ||||
50 | 3.9 | 100.9 | 2.1 | 99.5 | ||||
Codeine | 10 | 0.5-100 | 0.25 | 0.999 | 2.9 | 98.9 | 1.9 | 99.5 |
25 | 8.3 | 99.5 | 5.6 | 99.3 | ||||
50 | 2.4 | 99.3 | 1.5 | 9.8 | ||||
Clenbuterol | 10 | 0.5-100 | 0.25 | 0.9989 | 5.1 | 114.2 | 4.6 | 104.3 |
25 | 2.1 | 89.9 | 1.3 | 103.4 | ||||
50 | 1.2 | 99.9 | 1.3 | 105.6 | ||||
Salbutamol | 10 | 1-100 | 0.5 | 0.9998 | 2.1 | 99.9 | 1.9 | 102.3 |
25 | 1.9 | 98.4 | 1.8 | 101.2 | ||||
50 | 3.2 | 100.5 | 2.1 | 101.1 | ||||
Propranolol | 10 | 0.1-100 | 0.05 | 0.9999 | 2.2 | 99.7 | 1.9 | 100.5 |
25 | 2.6 | 89.9 | 2.5 | 99.78 | ||||
50 | 1.1 | 98.9 | 1.5 | 98.5 | ||||
Terbutaline | 10 | 0.5-100 | 0.25 | 0.9997 | 1.7 | 100.8 | 1.9 | 99.9 |
25 | 1.2 | 99.9 | 2.1 | 100.5 | ||||
50 | 2.2 | 100.5 | 1.9 | 102.3 | ||||
PCP | 10 | 0.5-100 | 0.25 | 0.9998 | 1.4 | 102.3 | 2 | 100.3 |
25 | 2.1 | 101.3 | 3.5 | 100.1 | ||||
50 | 3.2 | 105.1 | 1.5 | 100.5 | ||||
Ketamine | 10 | 0.5-100 | 0.25 | 0.9996 | 1.6 | 104.2 | 1.1 | 98.9 |
25 | 2.6 | 102.3 | 2.1 | 99.9 | ||||
50 | 2.4 | 104.2 | 2.2 | 99.6 | ||||