Figure 2

Mitochondrial depolarization mediated by triazole-containing alkyl β-D-glucopyranosides 4d and 4e and alkyl β-D-glucopyranosides C14G1. Jurkat cells were treated with triazole-containing alkyl β-D-glucopyranosides 4d and 4e and alkyl β-D-glucopyranosides C14G1 at their respective IC₅₀ concentration and incubated for 6 h. Changes in the mitochondrial ΔΨm was determined by staining with 2 μM of JC-1. The IC₅₀ concentration values that were used were the following: 4d = 53 μM; 4e = 24 μM; and C14G1 = 66.5 μM. After dissipation of ΔΨm, the JC-1 reagent emits a green fluorescence signal, while the compound in a polarizedmitochondrial membrane emits a red signal. Percentages of cells emitting green fluorescence signal (y-axis) are depicted. Each bar represents the mean ± SD of four independent replicates. The following controls were included: untreated cells as a negative control; cells treated with 0.1% v/v DMSO as a control for solvent effects; and cells exposed to 1 mM H₂O₂ as a positive control. Approximately 1x10⁴ flow cytometry events were acquired and analyzed per sample using CXP software.