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Table 1 pH Optimization of phosphate buffer

From: A validated stability-indicating HPLC method for the simultaneous determination of pheniramine maleate and naphazoline hydrochloride in pharmaceutical formulations

Mobile phase Theoretical plates (N) Symmetry Resolution (R) Peak shape
Methanol: Phosphate buffer, pH 2.8 (68:32)
Pheniramine 6762 0.91 7.75 +++
Naphazoline hydrochloride 6475 0.93 +++
Methanol: Phosphate buffer, pH 4 (68:32)
Pheniramine 1319 0.26 4.13 ___
Naphazoline hydrochloride 4737 0.43 +++
Methanol: Phosphate buffer, pH 5 (68:32)
Pheniramine 6731 0.54 0.98 +++
Naphazoline hydrochloride 1325 0.24 ___
Methanol: Phosphate buffer, pH 6 (68:32)
Pheniramine 6499 0.54 2.75 +++
Naphazoline hydrochloride 1683 0.23 ___