Table 1 pH Optimization of phosphate buffer
Mobile phase | Theoretical plates (N) | Symmetry | Resolution (R) | Peak shape |
|---|---|---|---|---|
Methanol: Phosphate buffer, pH 2.8 (68:32) | ||||
Pheniramine | 6762 | 0.91 | 7.75 | +++ |
Naphazoline hydrochloride | 6475 | 0.93 | +++ | |
Methanol: Phosphate buffer, pH 4 (68:32) | ||||
Pheniramine | 1319 | 0.26 | 4.13 | ___ |
Naphazoline hydrochloride | 4737 | 0.43 | +++ | |
Methanol: Phosphate buffer, pH 5 (68:32) | ||||
Pheniramine | 6731 | 0.54 | 0.98 | +++ |
Naphazoline hydrochloride | 1325 | 0.24 | ___ | |
Methanol: Phosphate buffer, pH 6 (68:32) | ||||
Pheniramine | 6499 | 0.54 | 2.75 | +++ |
Naphazoline hydrochloride | 1683 | 0.23 | ___ | |