Parameter | Number of theoretical plates (N) | Tailing factor | Resolution (Rs) | ||
---|---|---|---|---|---|
TS | JM | TS | JM | TS/JM | |
Organic modifier nature | |||||
Methanol | 7566 | 12598 | 1.06 | 1.10 | 11.10 |
1-Propanol | 5974 | 12154 | 1.16 | 0.20 | 16.40 |
Acetonitrile | 4253 | 9098 | 1.10 | 0.10 | 14.10 |
Methanol concentration (%) | |||||
8 | 3791 | 9496 | 1.05 | 1.09 | 9.97 |
10 | 5566 | 11598 | 1.06 | 1.10 | 11.10 |
12 | 4770 | 10204 | 1.17 | 1.24 | 11.42 |
14 | 7312 | 12723 | 1.09 | 1.10 | 11.16 |
16 | 4549 | 9675 | 1.21 | 1.31 | 11.80 |
SDS concentration (M) | |||||
0.10 | 4317 | 8931 | 1.20 | 1.24 | 12.72 |
0.12 | 5800 | 9848 | 1.20 | 1.28 | 12.07 |
0.15 | 5312 | 10723 | 1.21 | 1.21 | 11.67 |
0.17 | 7516 | 12973 | 1.09 | 1.13 | 10.81 |
0.20 | 3429 | 10814 | 1.19 | 1.05 | 10.76 |
pH | |||||
2.5 | 4247 | 9564 | 1.18 | 1.24 | 12.83 |
3.0 | 5985 | 10611 | 1.17 | 1.23 | 12.89 |
4.0 | 7516 | 12973 | 1.08 | 1.13 | 10.84 |
5.0 | 4830 | 11349 | 1.19 | 1.14 | 11.80 |