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Table 1 Optimization of experimental factors affecting the chromatographic performance of the proposed method

From: Simultaneous determination of tylosin and josamycin residues in muscles, liver, eggs and milk by MLC with a monolithic column and time-programmed UV detection: application to baby food and formulae

Parameter

Number of theoretical plates (N)

Tailing factor

Resolution (Rs)

 

TS

JM

TS

JM

TS/JM

Organic modifier nature

Methanol

7566

12598

1.06

1.10

11.10

1-Propanol

5974

12154

1.16

0.20

16.40

Acetonitrile

4253

9098

1.10

0.10

14.10

Methanol concentration (%)

8

3791

9496

1.05

1.09

9.97

10

5566

11598

1.06

1.10

11.10

12

4770

10204

1.17

1.24

11.42

14

7312

12723

1.09

1.10

11.16

16

4549

9675

1.21

1.31

11.80

SDS concentration (M)

0.10

4317

8931

1.20

1.24

12.72

0.12

5800

9848

1.20

1.28

12.07

0.15

5312

10723

1.21

1.21

11.67

0.17

7516

12973

1.09

1.13

10.81

0.20

3429

10814

1.19

1.05

10.76

pH

2.5

4247

9564

1.18

1.24

12.83

3.0

5985

10611

1.17

1.23

12.89

4.0

7516

12973

1.08

1.13

10.84

5.0

4830

11349

1.19

1.14

11.80