Figure 1
From: Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant
Comparison of HPLC analyses of the reaction components in a crude reaction mixture from the synthesis of N-lauroyl-N-methyl-glucamide by isocratic method using UV (A) and RI (B) detectors, and the developed gradient method using Evaporative Light Scattering Detector ELSD (C). Solutes: MEG: 1; amide: 2; lauric acid: 3; amide-ester: 4. Isocratic method: mobile phase: methanol: water: TFA 75:25:0.3 v/v ; flow rate 1 ml min−1 and UV wavelength set at 210 nm.