Figure 3

PAGE images of the native TBA and TBAs substituted with LNA (left) or OMeNA (right) in 50 mM KCl (upper) or 50 mM CaCl ₂ (lower) electrophoresis buffers, respectively. A, LNA-modified TBAs in 50 mM KCl (Ai) and 50 mM CaCl₂ (Aii); B, OMeNA-modified TBAs in 50 mM KCl (Bi) and 50 mM CaCl₂ (Bii). Native polyacrylamide gel electrophoresis was run in 50 mM KCl (upper) or 50 mM CaCl₂ (lower) electrophoresis buffers, respectively. Lanes 1 and 2, markers for 8 bp (C₄G₄*C₄G₄), and 16 bp (G₃TG₄AG₃TG₃*C₃AC₃TC₄AC₃) base-pair DNA duplexes, respectively; lane 3 and others, TBA and modified-TBAs loaded at 20 μM nucleoside concentrations, respectively.