Figure 5From: A validated spectrofluorimetric method for the determination of nifuroxazide through coumarin formation using experimental design Excitation and emission spectra of the coumarin product produced by reaction between 350 ng ml -1 of NF and EAA in methanol ( ____ ) and reagent blank (− − − -) (λ ex = 340 nm, λ em = 390 nm ). Back to article page