Enhanced recovery of alkaline protease from fish viscera by phase partitioning and its application

BMC Chemistry

Table 1 Two steps of aqueous two phase partitioning of alkaline protease from farmed giant catfish viscera

Step	Phase	Total activity (U)	Total protein (mg)	Specific activity (U/mg)	Purification (fold)	Recovery (%)
Crude extract^a	-	2,916.67±11.00^b	8.32±0.05	350.51±3.57	1.00	100
1st ATPS^c	Top	4,582.91±45.72	3.31±0.07	1,385.73±16.49	3.94±0.03	157.13±1.57
2nd ATPS^d	10	10,965.60±10.18	2.70±0.01	4,064.79±5.99	11.60±0.02	365.53±15.11

^a crude enzyme extract from farmed giant catfish viscera (1:5 (w/v) of the minced viscera to 10mM Tris–HCl buffer with 10 mM CaCl₂, pH 8.0).
^b values are given as mean ± S.D. from triplicate determinations.
^c 1st ATPS consists of 15% PEG2000-15%sodium citrate containing 70% (w/w) crude enzyme extract.
^d 2nd ATPS consists of the top phase of the second cycle ATPS, additional 10% (w/w) sodium citrate.

ISSN: 2661-801X