Figure 6
From: Enhanced recovery of alkaline protease from fish viscera by phase partitioning and its application
Peptide mapping of gelatin treated with the enzyme fraction (top phase) from the second cycle ATPS 15% PEG2000-15% sodium citrate containing 70% (w/w) crude enzyme extract with 10% additional sodium citrate (6A) and Flavourzyme (6B) [4% stacking gel and 7.5% separating gel SDS-PAGE]; M: molecular weight protein makers; C: control; 0.03, 0.1, 0.21, 0.50, 5, 10, 15, and 20 units: the unit of enzyme (initial enzyme 31.70 units); CE: crude enzyme extract.