Figure 5From: Enhanced recovery of alkaline protease from fish viscera by phase partitioning and its applicationSDS-PAGE patterns (5A) and activity staining (5B) performed using 4% stacking gel and 15% separating gel of alkaline protease from farmed giant catfish viscera 15% PEG2000-15% sodium citrate ATPS. [8 and 2 μg protein were loaded into the gel for protein patterns and activity staining, respectively.] Lane 1: crude enzyme extract; 2: top phase of effect CE; 3: top phase of effect pH; 4: top phase of effect NaCl addition; 5: top phase of repetitive ATPS; M: molecular weight protein makers.Back to article page