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Figure 5 | Chemistry Central Journal

Figure 5

From: Enhanced recovery of alkaline protease from fish viscera by phase partitioning and its application

Figure 5

SDS-PAGE patterns (5A) and activity staining (5B) performed using 4% stacking gel and 15% separating gel of alkaline protease from farmed giant catfish viscera 15% PEG2000-15% sodium citrate ATPS. [8 and 2 μg protein were loaded into the gel for protein patterns and activity staining, respectively.] Lane 1: crude enzyme extract; 2: top phase of effect CE; 3: top phase of effect pH; 4: top phase of effect NaCl addition; 5: top phase of repetitive ATPS; M: molecular weight protein makers.

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