Nominal conc. (ng/mL) | Run | Rabbit plasma | Human plasma | ||||
---|---|---|---|---|---|---|---|
Measured conc. (ng/mL ± SD) | Precision (CV,%) | Accuracy (recovery,%) | Measured conc. (ng/mL ± SD) | Precision (CV,%) | Accuracy (recovery,%) | ||
Intraday variation (six replicate at each concentration) | |||||||
0.26 | 1 | 0.27 ± 0.02 | 6.98 | 103 | 0.29 ± 0.01 | 3.60 | 110 |
2 | 0.26 ± 0.03 | 9.51 | 101 | 0.29 ± 0.03 | 9.61 | 109 | |
3 | 0.28 ± 0.03 | 12.2 | 108 | 0.30 ± 0.03 | 9.53 | 113 | |
1.28 | 1 | 1.30 ± 0.07 | 6.74 | 101 | 1.32 ± 0.08 | 5.80 | 103 |
2 | 1.32 ± 0.04 | 3.09 | 103 | 1.34 ± 0.05 | 3.51 | 105 | |
3 | 1.36 ± 0.06 | 4.78 | 106 | 1.37 ± 0.06 | 4.16 | 107 | |
64.0 | 1 | 59.3 ± 5.35 | 9.02 | 92.7 | 59.8 ± 5.95 | 9.94 | 93.5 |
2 | 64.8 ± 4.26 | 6.57 | 101 | 64.8 ± 5.42 | 8.36 | 101 | |
3 | 58.5 ± 6.19 | 10.60 | 91.4 | 63.8 ± 5.91 | 9.26 | 99.7 | |
800 | 1 | 832 ± 17.70 | 2.14 | 104 | 814 ± 43.60 | 5.36 | 102 |
2 | 778 ± 19.30 | 2.48 | 97.2 | 790 ± 34.50 | 4.37 | 98.8 | |
3 | 775.8 ± 38.60 | 4.97 | 97.0 | 769 ± 15.10 | 1.97 | 96.1 | |
Intraday variation (18 replicates at each concentration) | |||||||
0.26 | 0.27 ± 0.03 | 9.67 | 104 | 0.29 ± 0.03 | 9.72 | 111 | |
1.28 | 1.32 ± 0.06 | 4.80 | 103 | 1.34 ± 0.06 | 4.54 | 105 | |
64 | 60.9 ±5.80 | 9.49 | 95.1 | 62.9 ± 5.91 | 9.40 | 98.3 | |
800 | 795 ±36.80 | 4.62 | 99.4 | 791 ± 36.60 | 4.62 | 99.2 |