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Figure 1 | Chemistry Central Journal

Figure 1

From: Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model

Figure 1

Interference from HIU with the assay of absorbance at 308 nm after the lag time of 10 s for 0.30 mM uric acid under uricase action at 25°C. a. Effects of activities on interference in 0.10 M Tris–HCl buffer at pH 7.4 b. Effects of activities on interference in 0.20 M borate buffer at pH 7.4 c. Effects of reaction pH on interference in 0.10 M Tris–HCl buffer with final uricase activity at 103 U/L d. Effects of reaction pH on interference in 0.20 M borate buffer with final uricase activity at 103 U/L.

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